An ongoing outbreak of viral pneumonia was caused by a novel coronavirus in China in 2019. By March 19, over 200 thousand confirmed cases of SARS-CoV-2 infection and over 9000 deaths have been reported throughout the world.
For this infectious disease, nucleic acid detection is still the gold standard for pathogenic detection.
However, nucleic acid detection takes a long time and has relatively high “false negative”; therefore,Bio Med Frontierswe need urgently a convenient and accurate detection method to make up for this deficiency.
In this article, we will show such technical characteristics of lgM/lgG serum antibody detection, compared with nucleic acid detection.
Flavanol-Rich Cocoa Powder Interacts with Lactobacillus rhamnossus LGG to Alter the Antibody Response to Infection with the Parasitic Nematode Ascaris suum.
- Consumption of the probiotic bacteria LactobacillusrhamnosusLGG and flavanol-rich cocoa have purported immune modulating effects.
- This study compared the host response to infection with Ascaris suum in three-month-old pigs fed a standard growth diet supplemented with a vehicle control: LGG, cocoa powder (CP) or LGG + CP.
- Pigs were inoculated with infective A. suum eggs during Week 5 of dietary treatment and euthanized 17 days later.
- Lactobacillus abundance was increased in pigs fed LGG or LGG + CP. Specific anti-A. suum IgG2 antibodies were decreased (p < 0.05) in LGG + CP-fed pigs compared to pigs fed CP alone. Pigs fed LGG had significantly reduced expression (p < 0.05) of Eosinophil peroxidase (EPX), Interleukin 13 (IL-13), Eotaxin 3 (CCL26), Toll-like receptor 2 (TLR2), TLR4, and TLR9 and Interleukin-1Beta (IL1B) in the tracheal-bronchial lymph node (TBLN) independent of CP treatment.
- These results suggested that feeding LGG significantly reduced the localized prototypical Th2-related markers of infection with A. suum in the TBLN.
- Although feeding CP does not appear to affect the A. suum-induced Th2-associated cytokine response, feeding LGG + CP reduced anti-A. suum antibodies and delayed intestinal expulsion of parasitic larvae from the intestine.
Analysis of the relations between allergen specific LgG antibody and allergic dermatosis of 14 kinds foods.
- To use food-specific IgG antibody detection to explore its application in the allergy dermatoses. 181 patients were included from January 2014 to September 2014.
- Fourteen food-specific IgG antibodies were detected by ELISA. The positive rates of IgG antibody of the patient group and the healthy group were significantly different.
- The positive rates of IgG antibody of egg, milk, shrimp and crab took a large proportion in three groups of patients with three kinds of allergy dermatoses of urticaria, eczema and allergic dermatitis, the proportion of which was respectively 70.2%, 77.8% and 71.7%.
- There was mild and moderate intolerance of food in the allergic dermatitis group while there was no distribution difference of food intolerance in the urticaria group and eczema group.
- Among urticaria and allergic dermatitis patients with positive antibody, Supplier the positive rate of children was significantly higher than that of adults while there was no significant difference between children and adults among eczema patients with positive antibody.
- Allergy dermatoses are closely related to food-specific IgG antibody and the allergy dermatoses patients have a high incidence rate of food intolerance; detecting IgG antibody in patients is of great significance for the diagnosis and treatment of allergy dermatoses.
Qualification and performance characteristics of a quantitative enzyme-linked immunosorbent assay for human lgG antibodies to anthrax lethal factor antigen.
- The contribution of Bacillus anthracis lethal factor (LF)-specific immune responses to protection against anthrax disease in humans remains incompletely defined due, in part, to a paucity of qualified reagents and a lack of standardized serological assays.
- Toward this end, we have identified and characterized suitable positive quality control and standard reference sera and developed, optimized, and qualified an enzyme-linked immunosorbent assay (ELISA) to measure LF-binding IgG.
- Herein we describe the performance characteristics of this ELISA and propose criteria for its use in the detection and quantification of anti-LF IgG in human serum.
Enzyme immunoassay system for the detection of low-avid lgG antibodies to human cytomegalovirus (“CMV-diagnost”)
The new Russian enzyme immunoassay system “CMV-Diagnost” based on the detection of low-avid IgG antibodies has been developed for the rapid diagnosis of cytomegalovirus infection.
The system was found not only to determine the strained immunity in response to cytomegalovirus, but also to judge the current infection from the avidity index of detectable IgG antibodies with a high degree of validity.
The antibody avidity index of less than 30% suggests an acute stage of primary cytomegalovirus infection.
The minimum antibody threshold bodies (deltaOD has been established for the correct interpretation of data on low-avid antibodies. deltaOD of>> or =0.6 optic units was for the developed test system “CMV-Diagnost. A correlation was found between the serum levels of low-avid antibodies and IgM antibodies to cytomegalovirus at the acute stage of the disease.
Immune deconvolution and temporal mapping identifies stromal targets and developmental intervals for abrogating murine low-grade optic glioma formation
Brain tumor formation and progression are dictated by cooperative interactions between neoplastic and non-neoplastic cells.
This stromal dependence is nicely illustrated by tumors arising in the Neurofibromatosis type 1 (NF1) cancer predisposition syndrome, where children develop low-grade optic pathway gliomas (OPGs).
Using several authenticated Nf1-OPG murine models, we previously demonstrated that murine Nf1-OPG growth is regulated by T cell function and microglia Ccl5 production, such that their inhibition reduces tumor proliferation in vivo.
While these interactions are critical for established Nf1-OPG tumor growth, their importance in tumor formation has not been explored.
A combination of bulk and single-cell RNA mouse optic nerve sequencing, immunohistochemistry, T cell assays, and pharmacologic and antibody-mediated inhibition methods were used in these experiments.
We show that T cells and microglia are the main non-neoplastic immune cell populations in both murine and human LGGs.
Moreover, we demonstrate that CD8+ T cells, the predominant LGG-infiltrating lymphocyte population, are selectively recruited through increased Ccl2 receptor (Ccr4) expression in CD8+, but not CD4+, T cells, in a NF1/RAS-dependent manner.
Finally, we identify the times during gliomagenesis when microglia Ccl5 production (3-6 weeks of age) and Ccl2-mediated T cell infiltration (7-10 weeks of age) occur, such that temporally-restricted Ccl2 or Ccl5 inhibition abrogates tumor formation >3.5 months following the cessation of treatment.
Collectively, these findings provide proof-of-concept demonstrations that targeting stromal support during early gliomagenesis durably blocks murine LGG formation.
Escherichia coli Nissle 1917 Enhances Efficacy of Oral Attenuated Human Rotavirus Vaccine in a Gnotobiotic Piglet Model
Human rotavirus (HRV) infection is a major cause of viral gastroenteritis in young children worldwide. Current oral vaccines perform poorly in developing countries where efficacious vaccines are needed the most.
Therefore, an alternative affordable strategy to enhance efficacy of the current RV vaccines is necessary.
This study evaluated the effects of colonization of neonatal gnotobiotic (Gn) pigs with Escherichia coli Nissle (EcN) 1917 and Lacticaseibacillus rhamnosus GG (LGG) probiotics on immunogenicity and protective efficacy of oral attenuated (Att) HRV vaccine.
EcN-colonized pigs had reduced virulent HRV (VirHRV) shedding and decreased diarrhea severity compared with the LGG-colonized group.
Human Hepatitis C virus antibody lgG,HCV lgG ELISA KIT |
|||
| ED0173Hu | Jiaxing Korain Biotech Ltd (BT Labs) | 96 wells | 458 EUR |
Human Hepatitis C virus antibody lgG (HCV lgG) ELISA Kit |
|||
| MBS167627-10x96StripWells | MyBiosource | 10x96-Strip-Wells | 3375 EUR |
Human Hepatitis C virus antibody lgG (HCV lgG) ELISA Kit |
|||
| MBS167627-5x96StripWells | MyBiosource | 5x96-Strip-Wells | 1715 EUR |
Human Hepatitis C virus antibody lgG (HCV lgG) ELISA Kit |
|||
| MBS167627-96StripWells | MyBiosource | 96-Strip-Wells | 425 EUR |
Rat Legionella antibody lgG ELISA kit |
|||
| E02L0253-192T | BlueGene | 192 tests | 1524 EUR |
Rat Legionella antibody lgG ELISA kit |
|||
| E02L0253-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
Rat Legionella antibody lgG ELISA kit |
|||
| E02L0253-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
Pig Legionella antibody lgG ELISA kit |
|||
| E07L0253-192T | BlueGene | 192 tests | 1524 EUR |
Pig Legionella antibody lgG ELISA kit |
|||
| E07L0253-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
Pig Legionella antibody lgG ELISA kit |
|||
| E07L0253-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
Dog Legionella antibody lgG ELISA kit |
|||
| E08L0253-192T | BlueGene | 192 tests | 1524 EUR |
Dog Legionella antibody lgG ELISA kit |
|||
| E08L0253-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
Dog Legionella antibody lgG ELISA kit |
|||
| E08L0253-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
Rat Legionella antibody lgG ELISA Kit |
|||
| E01A14369 | BlueGene | 96T | 700 EUR |
Rat Legionella antibody lgG ELISA Kit |
|||
| MBS732377-10x96StripWells | MyBiosource | 10x96-Strip-Wells | 5685 EUR |
Rat Legionella antibody lgG ELISA Kit |
|||
| MBS732377-48StripWells | MyBiosource | 48-Strip-Wells | 485 EUR |
They also had enhanced HRV-specific IgA antibody titers in serum and antibody-secreting cell numbers in tissues pre/post VirHRV challenge, HRV-specific IgA antibody titers in intestinal contents, and B-cell subpopulations in tissues post VirHRV challenge.
EcN colonization also enhanced T-cell immune response, promoted dendritic cells and NK cell function, reduced production of proinflammatory cytokines/Toll like receptor (TLR), and increased production of immunoregulatory cytokines/TLR expression in various tissues pre/post VirHRV challenge.
Thus, EcN probiotic adjuvant with AttHRV vaccine enhances the immunogenicity and protective efficacy of AttHRV to a greater extent than LGG and it can be used as a safe and economical oral vaccine adjuvant.