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ONLY A SMALL FRACTION OF CELLS PRODUCE ASSEMBLED CAPSIDS DURING TRANSFECTION-BASED MANUFACTURING OF ADENO-ASSOCIATED VIRUS VECTORS
Plasmid transfection of mammalian cells is the dominant platform used to produce adeno-associated virus (AAV) vectors for clinical and research applications. Low yields from this platform currently make it difficult to supply these activities with adequate material. In an effort to better understand the current limitations of transfection-based manufacturing, this study examines what proportion of …
PROBLEMS OF REGULATORY AND LEGAL SUPPLY OF DRUGS CONTAINING NARCOTIC AND PSYCHOTROPIC SUBSTANCES OF OVERSEAS VESSEL
In the context of the COVID-19 pandemic, the issue of protecting the vital functions of crew members on ship’s board in conditions of a long voyage and stay in a confined space is urgent. In addition, excluding cases of infection with a new coronavirus infection, one must not forget about the readiness to provide first …
Upgrading DMEK Success by Identifying Optimal Levels of Trypan Blue Dye Application to Donor Corneal Tissue.
The reason for this study was to decide the right focus and span of use of trypan blue color to Descemet film endothelial keratoplasty (DMEK) benefactor corneal tissue before relocate to improve perceivability while limiting harm to the tissue. Corneas were precut for DMEK utilizing a 8-mm punch, and each was stained with either 0.06% …
SARS-CoV-2 lgM/lgG antibody detection confirms the infection after three negative nucleic acid detection
An ongoing outbreak of viral pneumonia was caused by a novel coronavirus in China in 2019. By March 19, over 200 thousand confirmed cases of SARS-CoV-2 infection and over 9000 deaths have been reported throughout the world. For this infectious disease, nucleic acid detection is still the gold standard for pathogenic detection. However, nucleic acid …
SARS-CoV-2 Antigen Variants
Rapid diagnostic tests (Ag-RDTs) for SARS-CoV-2 antigen detection provide laboratory-independent results at the point of care and are powerful tools for public health interventions. Clinical and analytical studies, published in 2021, showed detection thresholds for SARS-CoV-2 Ag-RDT related to the presence of infectious viruses in symptomatic SARS-CoV-2.1 infections, 2 However, most studies of Ag-RDT validation …
Spray-Dried Hierarchical Aggregates of Iron Oxide Nanoparticles and Their Functionalization for Downstream Processing in Biotechnology.
In this work, the structuring of iron oxide nanoparticles by way of spray-drying (SD) of aqueous suspensions is investigated, resulting in micrometer-sized aggregates with saturation magnetization corresponding to that of the person nanoparticles. Interestingly, the superparamagnetic conduct is retained regardless of the multicore construction. Modification of the aggregates by way of the addition of silica …
Resolving the formidable barrier of oxygen transferring rate (OTR) in ultrahigh-titer bioconversion/biocatalysis by a sealed-oxygen supply biotechnology (SOS).
The vital concern in the competitiveness between bioengineering and chemical engineering is the merchandise titer and the quantity productiveness. The most direct and efficient strategy normally employs high-density biocatalyst, whereas the weakened mass switch and evoked foam drawback accompany ultrahigh-density biocatalyst loading and substrate/product titer. In high-density obligate cardio bioconversion, oxygen as electron acceptor is …
Argentina’s Local Crop Biotechnology Developments: Why Have They Not Reached the Market Yet?
Plant biotechnology in Argentina started at the end of the 1980s, leading to the enchancment of fairly a number of evaluation groups in public institutions and, a decade later, to some native personal initiatives. The fairly a number of scientific and technological capacities current in the nation allowed the early construction in 1991 of a …
Argentina’s Local Crop Biotechnology Developments: Why Have They Not Reached the Market Yet?
Plant biotechnology in Argentina began at the finish of the 1980s, resulting in the improvement of quite a few analysis teams in public establishments and, a decade later, to some native personal initiatives. The quite a few scientific and technological capacities current in the nation allowed the early structure in 1991 of a sound genetically …