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ONLY A SMALL FRACTION OF CELLS PRODUCE ASSEMBLED CAPSIDS DURING TRANSFECTION-BASED MANUFACTURING OF ADENO-ASSOCIATED VIRUS VECTORS
Plasmid transfection of mammalian cells is the dominant platform used to produce adeno-associated virus (AAV) vectors for clinical and research applications. Low yields from this platform currently make it difficult to supply these activities with adequate material. In an effort to better understand the current limitations of transfection-based manufacturing, this study examines what proportion of …
PROBLEMS OF REGULATORY AND LEGAL SUPPLY OF DRUGS CONTAINING NARCOTIC AND PSYCHOTROPIC SUBSTANCES OF OVERSEAS VESSEL
In the context of the COVID-19 pandemic, the issue of protecting the vital functions of crew members on ship’s board in conditions of a long voyage and stay in a confined space is urgent. In addition, excluding cases of infection with a new coronavirus infection, one must not forget about the readiness to provide first …
Upgrading DMEK Success by Identifying Optimal Levels of Trypan Blue Dye Application to Donor Corneal Tissue.
The reason for this study was to decide the right focus and span of use of trypan blue color to Descemet film endothelial keratoplasty (DMEK) benefactor corneal tissue before relocate to improve perceivability while limiting harm to the tissue. Corneas were precut for DMEK utilizing a 8-mm punch, and each was stained with either 0.06% …
SARS-CoV-2 lgM/lgG antibody detection confirms the infection after three negative nucleic acid detection
An ongoing outbreak of viral pneumonia was caused by a novel coronavirus in China in 2019. By March 19, over 200 thousand confirmed cases of SARS-CoV-2 infection and over 9000 deaths have been reported throughout the world. For this infectious disease, nucleic acid detection is still the gold standard for pathogenic detection. However, nucleic acid …