Resolving the formidable barrier of oxygen transferring rate (OTR) in ultrahigh-titer bioconversion/biocatalysis by a sealed-oxygen supply biotechnology (SOS).

Resolving the formidable barrier of oxygen transferring rate (OTR) in ultrahigh-titer bioconversion/biocatalysis by a sealed-oxygen supply biotechnology (SOS).

The vital concern in the competitiveness between bioengineering and chemical engineering is the merchandise titer and the quantity productiveness. The most direct and efficient strategy normally employs high-density biocatalyst, whereas the weakened mass switch and evoked foam drawback accompany ultrahigh-density biocatalyst loading and substrate/product titer.

In high-density obligate cardio bioconversion, oxygen as electron acceptor is a speed-limiting step in bioprocesses, however enough oxygen supply will result in the foaming which ends in a important discount in oxygen utilization and the use of further defoamers. In this examine, we designed a novel sealed-oxygen supply (SOS) biotechnology to resolve the formidable barrier of oxygen transferring rate (OTR), for bio-based fuels and chemical manufacturing course of.

Incorporating male sterility into hybrid seed manufacturing reduces its price and ensures excessive varietal purity. Despite these benefits, male-sterile traces haven’t been broadly used to provide tomato (Solanum lycopersicum) hybrid seeds. We describe the improvement of a biotechnology-based breeding platform that utilized genic male sterility to provide hybrid seeds. In this platform, we generated a novel male-sterile tomato line by clustered usually interspaced brief palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated mutagenesis of a stamen-specific gene SlSTR1 and devised a transgenic maintainer by reworking male-sterile vegetation with a fertility-restoration gene linked to a seedling-colour gene.

Offspring of crosses between a hemizygous maintainer and the homozygous male-sterile plant segregated into 50% non-transgenic male-sterile vegetation and 50% male-fertile maintainer vegetation, which may very well be simply distinguished by seedling color. This system has nice sensible potential for hybrid seed breeding and manufacturing because it overcomes the issues intrinsic to different male sterility programs and could be simply tailored for a vary of tomato cultivars and numerous vegetable crops.

DAPI Dilactate

17509 25 mg
EUR 158.4

DAPI (hydrochloride)

C3362-10 10 mg
EUR 146.4

DAPI (hydrochloride)

C3362-25 25 mg
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DAPI (hydrochloride)

C3362-5 5 mg
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DAPI (dihydrochloride)

HY-D0814 50mg
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Arium Dual Wavelength UV Lamp

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DAPI Staining Kit

abx097206-1Kit 1 Kit
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FluorLast? with DAPI

1214-20 each
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DAPI (Molecular Biology Grade)

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DAPI (Molecular Biology Grade)

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DAPI (Molecular Biology Grade)

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FluorLast? with DAPI and DABCO

1218-20 each
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FluoroQuest™ Mounting Medium with DAPI

20004 50 mL
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EverBrite Hardset Mounting Medium with DAPI

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Description: Minimum order quantity: 1 unit of 10mL

Anti-Fade Mounting Medium (With DAPI)

abx090690-10ml 10 ml
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DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride)

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FluoroQuest™ Anti-fading Mounting Medium with DAPI

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DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*

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DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*

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DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*

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Human 4’,6-Diamidino-2-phenylindole dihydrochloride(DAPI)ELISA kit

QY-E05538 96T
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DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *10 mM solution in water*

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DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) for nucleic acid staining

AR1176 10mL(100 assys)
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DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) for nucleic acid staining

AR1177 50mL(500 assys)
EUR 121.2

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