Resolving the formidable barrier of oxygen transferring rate (OTR) in ultrahigh-titer bioconversion/biocatalysis by a sealed-oxygen supply biotechnology (SOS).

Resolving the formidable barrier of oxygen transferring rate (OTR) in ultrahigh-titer bioconversion/biocatalysis by a sealed-oxygen supply biotechnology (SOS).

Leave a Comment / Resolving the formidable barrier of oxygen transferring rate (OTR) in ultrahigh-titer bioconversion/biocatalysis by a sealed-oxygen supply biotechnology (SOS). / By

The vital concern in the competitiveness between bioengineering and chemical engineering is the merchandise titer and the quantity productiveness. The most direct and efficient strategy normally employs high-density biocatalyst, whereas the weakened mass switch and evoked foam drawback accompany ultrahigh-density biocatalyst loading and substrate/product titer.

In high-density obligate cardio bioconversion, oxygen as electron acceptor is a speed-limiting step in bioprocesses, however enough oxygen supply will result in the foaming which ends in a important discount in oxygen utilization and the use of further defoamers. In this examine, we designed a novel sealed-oxygen supply (SOS) biotechnology to resolve the formidable barrier of oxygen transferring rate (OTR), for bio-based fuels and chemical manufacturing course of.

Incorporating male sterility into hybrid seed manufacturing reduces its price and ensures excessive varietal purity. Despite these benefits, male-sterile traces haven’t been broadly used to provide tomato (Solanum lycopersicum) hybrid seeds. We describe the improvement of a biotechnology-based breeding platform that utilized genic male sterility to provide hybrid seeds. In this platform, we generated a novel male-sterile tomato line by clustered usually interspaced brief palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated mutagenesis of a stamen-specific gene SlSTR1 and devised a transgenic maintainer by reworking male-sterile vegetation with a fertility-restoration gene linked to a seedling-colour gene.

Offspring of crosses between a hemizygous maintainer and the homozygous male-sterile plant segregated into 50% non-transgenic male-sterile vegetation and 50% male-fertile maintainer vegetation, which may very well be simply distinguished by seedling color. This system has nice sensible potential for hybrid seed breeding and manufacturing because it overcomes the issues intrinsic to different male sterility programs and could be simply tailored for a vary of tomato cultivars and numerous vegetable crops.

Ultraviolet wavelength-sensitive opsin Polyclonal Antibody
MBS9233874-01mL 0.1mL
EUR 415

Ultraviolet wavelength-sensitive opsin Polyclonal Antibody
MBS9233874-5x01mL 5x0.1mL
EUR 1841

AgileReader TM any filters wavelength from 340nm to 900nm can be customized
AMPR-900-filter each Ask for price

Orion AQUAfast IV Colorimeter 4 wavelengths and auto ID - EACH
AQ4000 EACH
EUR 2026.35

Orion AQUAfast Colorimeter 6 wavelengths for COD and other digestion methods - EACH
AQ3700 EACH
EUR 2373.3

DAPI
40011-1 EA
EUR 122

DAPI
abx283157-100g 100 µg Ask for price

DAPI
abx283157-20g 20 µg
EUR 106.25

DAPI
abx283157-50g 50 µg
EUR 237.5

DAPI
B1098-25 each
EUR 496.8

DAPI
B1098-5 each
EUR 170.4

DAPI
BMD0063-100mg 100 mg
EUR 849
Description: DAPI is a popular blue fluorescent DNA dye.

DAPI
BMD0063-10mg 10 mg
EUR 119
Description: DAPI is a popular blue fluorescent DNA dye.

DAPI
BMD0063-each each Ask for price

DAPI
abx082063-100l 100 µl
EUR 212.5

DAPI
abx082063-10mg 10 mg
EUR 276

DAPI
abx082063-1ml 1 ml Ask for price

DAPI
abx082063-200l 200 µl Ask for price

DAPI
MBS9719177-100mg 100mg
EUR 605

DAPI
MBS9719177-10mg 10mg
EUR 155

DAPI
MBS9719177-5x100mg 5x100mg
EUR 2685

DAPI Reagent
MBS2567122-100Tests 100Tests
EUR 85

DAPI Reagent
MBS2567122-200Tests 200Tests
EUR 95

DAPI Reagent
MBS2567122-500Tests 500Tests
EUR 115

DAPI Reagent
MBS2567122-50Tests 50Tests
EUR 80

DAPI Reagent
MBS2567122-5x500Tests 5x500Tests
EUR 510

DAPI Reagent
MBS2563514-10mL 10mL
EUR 100

DAPI Reagent
MBS2563514-50mL 50mL
EUR 150

DAPI Reagent
MBS2563514-5mL 5mL
EUR 90

DAPI Reagent
MBS2563514-5x50mL 5x50mL
EUR 640

DAPI Dilactate
17509 25 mg
EUR 112
Description: DAPI is a fluorescent stain that binds strongly to DNA.

DAPI Dilactate
17509-25mg 25 mg
EUR 109
Description: DAPI is a fluorescent stain that binds strongly to DNA.

DAPI (hydrochloride)
C3362-10 10 mg
EUR 64
Description: Probes & Dyes|Nuclear Stains

DAPI (hydrochloride)
C3362-25 25 mg
EUR 130.4
Description: Probes & Dyes|Nuclear Stains

DAPI (hydrochloride)
C3362-5 5 mg
EUR 40
Description: Probes & Dyes|Nuclear Stains

DAPI (Hydrochloride)
MBS696332-25mg 25mg
EUR 260

DAPI (Hydrochloride)
MBS696332-5mg 5mg
EUR 150

DAPI (Hydrochloride)
MBS696332-5x25mg 5x25mg
EUR 1120

DAPI Dihydrochloride
561706 5.0mg
EUR 190

DAPI (dihydrochloride)
HY-D0814 50mg
EUR 439.2

DAPI dihydrochloride
MB097-10MG 1 unit
EUR 41.98
Description: DAPI dihydrochloride

DAPI dihydrochloride
MB097-25MG 1 unit
EUR 83.31
Description: DAPI dihydrochloride

DAPI Dihydrochloride
MBS5756233-100mg 100mg
EUR 390

DAPI Dihydrochloride
MBS5756233-10mg 10mg
EUR 165

DAPI Dihydrochloride
MBS5756233-25mg 25mg
EUR 225

DAPI Dihydrochloride
MBS5756233-50mg 50mg
EUR 315

DAPI Dihydrochloride
MBS5756233-5mg 5mg
EUR 155

DAPI Dihydrochloride
T19827-10mg 10mg Ask for price
Description: DAPI Dihydrochloride

DAPI Dihydrochloride
T19827-1g 1g Ask for price
Description: DAPI Dihydrochloride

DAPI Dihydrochloride
T19827-1mg 1mg Ask for price
Description: DAPI Dihydrochloride

DAPI Dihydrochloride
T19827-50mg 50mg Ask for price
Description: DAPI Dihydrochloride

DAPI Dihydrochloride
T19827-5mg 5mg Ask for price
Description: DAPI Dihydrochloride

DAPI dihydrochloride
RM5773-25MG 1 unit
EUR 119.85
Description: DAPI dihydrochloride

DAPI Staining Kit
abx097206-100l 100 µl
EUR 162.5

DAPI Staining Kit
abx097206-1Kit 1 Kit
EUR 276

DAPI Staining Kit
abx097206-1ml 1 ml Ask for price

DAPI Staining Kit
abx097206-200l 200 µl Ask for price

DAPI Staining Kit
MBS826728-01mL 0.1mL
EUR 140

DAPI Staining Kit
MBS826728-5x01mL 5x0.1mL
EUR 550

DAPI solution（0.25mM）
E37F17510-50-mL 50 mL
EUR 153
Description: Cell staining. Nuclear staining.

DAPI solution（0.25mM）
E37F17510-5x10-mL 5×10 mL
EUR 173
Description: Cell staining. Nuclear staining.

FluorLast? with DAPI
1214-20 each
EUR 183.6

DAPI Staining Solution
MBS176580-10mL 10mL
EUR 115

DAPI Staining Solution
MBS176580-50mL 50mL
EUR 135

DAPI Staining Solution
MBS176580-5x50mL 5x50mL
EUR 450

Dapi-Fluoromount-G
MBS679125-20mL 20mL
EUR 150

Dapi-Fluoromount-G
MBS679125-5x20mL 5x20mL
EUR 535

DAPI Reference Standard
BLI906A-1 1ml
EUR 303.48

DAPI Reference Standard
BLI906B-5 5ml
EUR 598.32

DAPI Reference Standard
BLI906C-14 2x7ml
EUR 1153.44

DAPI Reagent (25μg/mL)
E-CK-A163-100T 100T
EUR 15

DAPI Reagent (25μg/mL)
E-CK-A163-200T 200T
EUR 25

DAPI Reagent (25μg/mL)
E-CK-A163-500T 500T
EUR 55

DAPI Reagent (25μg/mL)
E-CK-A163-50T 50T
EUR 10

DAPI Solution(1mg/mL)
19178-91 1ML
EUR 38.5

DAPI Reagent (25ug/mL)
E-CK-A163-100Tests 100Tests
EUR 15

DAPI Reagent (25ug/mL)
E-CK-A163-200Tests 200Tests
EUR 25

DAPI Reagent (25ug/mL)
E-CK-A163-500Tests 500Tests
EUR 55

DAPI Reagent (25ug/mL)
E-CK-A163-50Tests 50Tests
EUR 10

DAPI solution（1mg/ml）
E37F17510DZ-1-mL 1 mL
EUR 60
Description: Cell staining. Nuclear staining.

DAPI Led Filter Cube
LBI10101 each
EUR 2735.9

DAPI solution (1mg/ml)
MBS8579913-1mL 1mL
EUR 140

DAPI solution (1mg/ml)
MBS8579913-5x1mL 5x1mL
EUR 570

DAPI-solution（1mg/ml）
E37F17510DZ 1-mL
EUR 60
Description: Cell-staining.-Nuclear-staining.

Fluoroshieldâ„¢ with DAPI
AR-6501-01 20 ml
EUR 68.05

Fluoroshieldâ„¢ with DAPI
AR-6501-02 100 ml
EUR 247.1

Fluoroshield with DAPI & PPD
AR-6513-01 20 ml
EUR 68.05

Fluoroshield with DAPI & PPD
AR-6513-02 100 ml
EUR 231

DAPI (Molecular Biology Grade)
CE117 5 mg
EUR 40

DAPI (Molecular Biology Grade)
CE118 25 mg
EUR 133

DAPI (Molecular Biology Grade)
CE119 100 mg
EUR 281

Fluoroshield with DAPI & DABCO
AR-6505-01 20 ml
EUR 68.05

Fluoroshield with DAPI & DABCO
AR-6505-02 100 ml
EUR 247.1

DAPI, 10mg/mL in H2O
40043-1 EA
EUR 103

DAPI Reagent (1 ug/mL)
E-IR-R103-10mL 10mL
EUR 35

DAPI Reagent (1 ug/mL)
E-IR-R103-50mL 50mL
EUR 105

DAPI Reagent (1 ug/mL)
E-IR-R103-5mL 5mL
EUR 20

DAPI Reagent (1 ug/mL)
E-IR-R103-each each Ask for price

DAPI (Ex375/28, Em460/50)
LBI10130 each
EUR 3270

Fluoroshield with DAPI & Propylgallate
AR-6506-01 20 ml
EUR 68.05

Fluoroshield with DAPI & Propylgallate
AR-6506-02 100 ml
EUR 247.1

FluorLast? with DAPI and DABCO
1218-20 each
EUR 183.6

Fluoromount Mounting Medium with DAPI
42734051-1 20 mL
EUR 98.6

FluoroQuestâ„¢ Mounting Medium with DAPI
20004-50mL 50 mL
EUR 109
Description: FluoroQuest™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

FluoroQuestâ„¢ Mounting Medium with DAPI
20004 50 mL
EUR 112
Description: FluoroQuest™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

OOSB00007-20ML - Dapi-Fluoromount-G
OOSB00007-20ML 20mL
EUR 79

DAPI staining kit (ready to use) 
EGY0621 10mL
EUR 85

DAPI staining kit (ready to use) 
EGY0622 50mL
EUR 185

DAPI staining kit (ready to use) 
MBS8584952-10mL 10mL
EUR 200

DAPI staining kit (ready to use) 
MBS8584952-5x10mL 5x10mL
EUR 915

DAPI staining kit (ready to use) 
MBS8584953-50mL 50mL
EUR 285

DAPI staining kit (ready to use) 
MBS8584953-5x50mL 5x50mL
EUR 1300

Annexin V-PE/DAPI Apoptosis Kit
E-CK-A256-100Assays 100 Assays
EUR 260
Description: Apoptosis/Annexin V

Annexin V-PE/DAPI Apoptosis Kit
E-CK-A256-200Assays 200 Assays
EUR 398
Description: Apoptosis/Annexin V

Annexin V-PE/DAPI Apoptosis Kit
E-CK-A256-20Assays 20 Assays
EUR 75
Description: Apoptosis/Annexin V

Annexin V-PE/DAPI Apoptosis Kit
E-CK-A256-50Assays 50 Assays
EUR 150
Description: Apoptosis/Annexin V

Annexin V-APC/DAPI Apoptosis Kit
E-CK-A258-100Assays 100 Assays
EUR 260
Description: Apoptosis/Annexin V

Annexin V-APC/DAPI Apoptosis Kit
E-CK-A258-200Assays 200 Assays
EUR 398
Description: Apoptosis/Annexin V

Annexin V-APC/DAPI Apoptosis Kit
E-CK-A258-20Assays 20 Assays
EUR 75
Description: Apoptosis/Annexin V

Annexin V-APC/DAPI Apoptosis Kit
E-CK-A258-50Assays 50 Assays
EUR 150
Description: Apoptosis/Annexin V

Annexin V-FITC/DAPI Apoptosis Kit
E-CK-A252-100Assays 100 Assays
EUR 260
Description: Apoptosis/Annexin V

Annexin V-FITC/DAPI Apoptosis Kit
E-CK-A252-200Assays 200 Assays
EUR 398
Description: Apoptosis/Annexin V

Annexin V-FITC/DAPI Apoptosis Kit
E-CK-A252-20Assays 20 Assays
EUR 75
Description: Apoptosis/Annexin V

Annexin V-FITC/DAPI Apoptosis Kit
E-CK-A252-50Assays 50 Assays
EUR 150
Description: Apoptosis/Annexin V

Annexin V-EGFP/DAPI Apoptosis Kit
E-CK-A260-100Assays 100 Assays
EUR 260
Description: Apoptosis/Annexin V

Annexin V-EGFP/DAPI Apoptosis Kit
E-CK-A260-200Assays 200 Assays
EUR 398
Description: Apoptosis/Annexin V

Annexin V-EGFP/DAPI Apoptosis Kit
E-CK-A260-20Assays 20 Assays
EUR 75
Description: Apoptosis/Annexin V

Annexin V-EGFP/DAPI Apoptosis Kit
E-CK-A260-50Assays 50 Assays
EUR 150
Description: Apoptosis/Annexin V

DAPI blue fluorescent nuclear DNA dye
6244 10 mg
EUR 120.5

Anti-Fade Mounting Medium (With DAPI)
abx090690-100l 100 µl
EUR 137.5

Anti-Fade Mounting Medium (With DAPI)
abx090690-10ml 10 ml
EUR 243.6

Anti-Fade Mounting Medium (With DAPI)
abx090690-1ml 1 ml Ask for price

Anti-Fade Mounting Medium (With DAPI)
abx090690-200l 200 µl Ask for price

Anti-fade Mounting Medium (With DAPI)
MBS355738-10mL 10mL
EUR 180

Anti-fade Mounting Medium (With DAPI)
MBS355738-5x10mL 5x10mL
EUR 515

DAPI blue fluorescent nuclear DNA dye
MBS258073-10mg 10mg
EUR 245

DAPI blue fluorescent nuclear DNA dye
MBS258073-5x10mg 5x10mg
EUR 930

EverBriteâ„¢ Mounting Medium with DAPI
23002-1 EA
EUR 172

Annexin V-Cyanine5/DAPI Apoptosis Kit
E-CK-A262-100Assays 100 Assays
EUR 260
Description: Apoptosis/Annexin V

Annexin V-Cyanine5/DAPI Apoptosis Kit
E-CK-A262-200Assays 200 Assays
EUR 398
Description: Apoptosis/Annexin V

Annexin V-Cyanine5/DAPI Apoptosis Kit
E-CK-A262-20Assays 20 Assays
EUR 75
Description: Apoptosis/Annexin V

Annexin V-Cyanine5/DAPI Apoptosis Kit
E-CK-A262-50Assays 50 Assays
EUR 150
Description: Apoptosis/Annexin V

DAPI in Fluorguard™ (0.3 µg/ml)
F201 1 ml Ask for price

DAPI in Fluorguard™ (0.5 µg/ml)
F203 1 ml Ask for price

DAPI in Fluorguard™ (1 µg/ml)
F204 1 ml Ask for price

Enhanced Antifade Mounting Medium with DAPI
MBS9719255-10mL 10mL
EUR 115

Enhanced Antifade Mounting Medium with DAPI
MBS9719255-50mL 50mL
EUR 140

Enhanced Antifade Mounting Medium with DAPI
MBS9719255-5x10mL 5x10mL
EUR 500

Enhanced Antifade Mounting Medium with DAPI
MBS9719255-5x50mL 5x50mL
EUR 615

Immuno Fluoro Mount with DABCO and DAPI
AR-6519-01 20 ml
EUR 68.05

Immuno Fluoro Mount with DABCO and DAPI
AR-6519-02 100 ml
EUR 207.2

Annexin V-PE/Cyanine5/DAPI Apoptosis Kit
E-CK-A264-100Assays 100 Assays
EUR 260
Description: Apoptosis/Annexin V

Annexin V-PE/Cyanine5/DAPI Apoptosis Kit
E-CK-A264-200Assays 200 Assays
EUR 398
Description: Apoptosis/Annexin V

Annexin V-PE/Cyanine5/DAPI Apoptosis Kit
E-CK-A264-20Assays 20 Assays
EUR 75
Description: Apoptosis/Annexin V

Annexin V-PE/Cyanine5/DAPI Apoptosis Kit
E-CK-A264-50Assays 50 Assays
EUR 150
Description: Apoptosis/Annexin V

Annexin V-PE/Cyanine5.5/DAPI Apoptosis Kit
E-CK-A266-100Assays 100 Assays
EUR 260
Description: Apoptosis/Annexin V

Annexin V-PE/Cyanine5.5/DAPI Apoptosis Kit
E-CK-A266-200Assays 200 Assays
EUR 398
Description: Apoptosis/Annexin V

Annexin V-PE/Cyanine5.5/DAPI Apoptosis Kit
E-CK-A266-20Assays 20 Assays
EUR 75
Description: Apoptosis/Annexin V

Annexin V-PE/Cyanine5.5/DAPI Apoptosis Kit
E-CK-A266-50Assays 50 Assays
EUR 150
Description: Apoptosis/Annexin V

Annexin V-PE/Cyanine7/DAPI Apoptosis Kit
E-CK-A268-100Assays 100 Assays
EUR 260
Description: Apoptosis/Annexin V

Annexin V-PE/Cyanine7/DAPI Apoptosis Kit
E-CK-A268-200Assays 200 Assays
EUR 398
Description: Apoptosis/Annexin V

Annexin V-PE/Cyanine7/DAPI Apoptosis Kit
E-CK-A268-20Assays 20 Assays
EUR 75
Description: Apoptosis/Annexin V

Annexin V-PE/Cyanine7/DAPI Apoptosis Kit
E-CK-A268-50Assays 50 Assays
EUR 150
Description: Apoptosis/Annexin V

Annexin V-APC/Cyanine7/DAPI Apoptosis Kit
E-CK-A270-100Assays 100 Assays
EUR 260
Description: Apoptosis/Annexin V

Annexin V-APC/Cyanine7/DAPI Apoptosis Kit
E-CK-A270-200Assays 200 Assays
EUR 398
Description: Apoptosis/Annexin V

Annexin V-APC/Cyanine7/DAPI Apoptosis Kit
E-CK-A270-20Assays 20 Assays
EUR 75
Description: Apoptosis/Annexin V

Annexin V-APC/Cyanine7/DAPI Apoptosis Kit
E-CK-A270-50Assays 50 Assays
EUR 150
Description: Apoptosis/Annexin V

Fluorescent Mounting Medium with DAPI, 18 ml
E19-18 -
EUR 125

Annexin V-PE/DAPI Apoptosis Detection Kit
MBS2567095-100Assays 100Assays
EUR 310

Annexin V-PE/DAPI Apoptosis Detection Kit
MBS2567095-200Assays 200Assays
EUR 395

Annexin V-PE/DAPI Apoptosis Detection Kit
MBS2567095-20Assays 20Assays
EUR 195

Annexin V-PE/DAPI Apoptosis Detection Kit
MBS2567095-50Assays 50Assays
EUR 240

Annexin V-PE/DAPI Apoptosis Detection Kit
MBS2567095-5x200Assays 5x200Assays
EUR 1800

Glycerol mounting medium with DAPI and DABCO
AR-6510-01 20 ml
EUR 68.05

Glycerol mounting medium with DAPI and DABCO
AR-6510-02 100 ml
EUR 207.2

Annexin V-APC/DAPI Apoptosis Detection Kit
MBS2567096-100Assays 100Assays
EUR 310

Annexin V-APC/DAPI Apoptosis Detection Kit
MBS2567096-200Assays 200Assays
EUR 395

Annexin V-APC/DAPI Apoptosis Detection Kit
MBS2567096-20Assays 20Assays
EUR 195

Annexin V-APC/DAPI Apoptosis Detection Kit
MBS2567096-50Assays 50Assays
EUR 240

Annexin V-APC/DAPI Apoptosis Detection Kit
MBS2567096-5x200Assays 5x200Assays
EUR 1800

Annexin V-FITC/DAPI Apoptosis Detection Kit
MBS2567093-100Assays 100Assays
EUR 310

Annexin V-FITC/DAPI Apoptosis Detection Kit
MBS2567093-200Assays 200Assays
EUR 395

Annexin V-FITC/DAPI Apoptosis Detection Kit
MBS2567093-20Assays 20Assays
EUR 195

Annexin V-FITC/DAPI Apoptosis Detection Kit
MBS2567093-50Assays 50Assays
EUR 240

Annexin V-FITC/DAPI Apoptosis Detection Kit
MBS2567093-5x200Assays 5x200Assays
EUR 1800

Annexin V-EGFP/DAPI Apoptosis Detection Kit
MBS2567097-100Assays 100Assays
EUR 310

Annexin V-EGFP/DAPI Apoptosis Detection Kit
MBS2567097-200Assays 200Assays
EUR 395

Annexin V-EGFP/DAPI Apoptosis Detection Kit
MBS2567097-20Assays 20Assays
EUR 195

Annexin V-EGFP/DAPI Apoptosis Detection Kit
MBS2567097-50Assays 50Assays
EUR 240

Annexin V-EGFP/DAPI Apoptosis Detection Kit
MBS2567097-5x200Assays 5x200Assays
EUR 1800

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